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KMID : 1025520060480010001
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Journal of Animal Science and Technology
2006 Volume.48 No. 1 p.1 ~ p.8
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Novel Mutation and Genetic Variation of the KIT Gene in Korean Wild Boars(Sus scrofa coreanus)
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Cho In-Cheol
Choi Y. L.
Ko Moon-Suck
Kim J.-H.
Lee Jung-Gyu
Jeon Jin-Tae
Lee Hang
Oh Moon-You
Han Sang-Hyun
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Abstract
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KIT encodes a mast/stem cell growth factor receptor and is known as a possible candidate gene responsible for dominant white coat color in mammals. To investigate the genetic variation of KIT gene in Korean wild boars(Sus scrofa coreanus), we carried out PCR-RFLP and DNA sequencing for three exons(exons 17, 19, and 20) and intron 19 of the KIT gene in Korean wild boars. PCR-RFLP results using NlaⅢ restriction enzyme in the breakpoint region between exon 17 and intron 17 and AciⅠ restriction enzyme in exon 19 indicate that Korean wild boars did not have previously identified white coat color related splicing mutation and missense mutation, respectively. These results also indicate matings between Korean wild boars could not give white coat color offsprings. We also found new SNPs in exons 19(C2661T) and 20(A2760G). Of these, the SNP in exon 20 is a missense mutation which might induce the change of amino acid iso-leucine to valine. However, no relationship was identified with this missense mutation and coat color. In this study, breed specific new SNPs were identified in exons 19, 20 and intron 19 and these results will give important information for genetic variation of porcine KIT gene.
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KEYWORD
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Coat color, KIT, Mutation, Pig, RFLP, Wild boar
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